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【Objective:】 To understand the development of narrative medicine in medical schools and the cognitive level of narrative medicine among medical students. 【Methods:】 Taking 2,353 medical students nationwide as the research subjects, Knowledge, Attitude/Belief, Practice (KAP) questionnaire was distributed online to conduct a survey. The survey results were analyzed from the perspectives of general knowledge, ways to understand narrative medicine, and cognitive level of parallel medical records. 【Results:】 44.50% of the survey respondents had never learned about narrative medicine, and 50.79% of them had never heard of parallel medical records. 26.80% of the survey respondents had participated in the training of narrative medicine courses, while most medical students learned about narrative medicine through other ways. 【Conclusion:】 This paper showed that the majority of the survey respondents are not familiar with the key concepts of narrative medicine. By introducing the concept of big culture in anthropology into medical college education or exploring the perspective of doctor-patient interaction, integrating communication models to improve doctor-patient relationships. The current popularization level of parallel medical records and narrative types among medical students is not good, and the cultivation of cultural sensitivity and reflective ability may help promote the teaching and application of parallel medical records. Multiple learning pathways lay the foundation for further teaching, and build a diversified teaching system to enrich the course content, which should be the focus of promoting narrative medicine education in the future.
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Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system. However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1. Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive. In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly. Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively. It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression. Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1. Significantly, Ginsenoside Rb1 loaded on micro-nano HAp/silk could facilitate osteogenesis and angiogenesis. All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor's expression of BMSCs. Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.
Subject(s)
Animals , Rats , Alginates/pharmacology , Bone Regeneration , Cell Differentiation , Durapatite/pharmacology , Ginsenosides , Osteogenesis , Silk/pharmacology , Tissue ScaffoldsABSTRACT
Objective@#This study aimed to analyze the relationship between cerebral ischemia and cerebral blood flow dynamics (cerebrovascular function score), related physiological, and biochemical indexes.@*Methods@#We selected 366 patients who underwent regular physical examination, cerebrovascular hemodynamics test, and head MRI in the health management center of Shandong Electric Power Central Hospital from May 2016 to April 2019. The patients with cerebral ischemia were selected as the case group (264 cases), and those without cerebral ischemia as the control group (102 cases) to analyze the differences in cerebrovascular function scores, blood pressure, body mass index, and related biochemical indexes between the two groups. The risk factors were also analyzed by multiple logistic regression analysis.@*Results@#The average age of cerebral the ischemia group was (53.0±7.6) years, which consisted of 211 men and 53 women. The average age of the control group was (48.7±7.3) years, which consisted of 87 men and 15 women. The comparison of the mean of continuous variables between the two groups of subjects shows that the mean age [(53.0±7.6) years old vs. (48.7±7.3) years old] and systolic blood pressure [(127.70±17.06) mmHg vs. (122.36±14.75) mmHg] (1 mmHg=0.133 kPa) of cerebral ischemia group were significantly higher than that of control group. The high-density lipoprotein level [(1.29±0.26) mmol/L vs. (1.37±0.30) mmol/L] and cerebrovascular function score [(86.55±18.41) vs. （92.16±10.77）] were significantly lower than those in the control group. The exposure rate of systolic blood pressure ≥140 mmHg and cerebrovascular function score <75 [16.7%(44 cases) vs. 2.9%(3 cases)] in the ischemic group was significantly higher than that in the control group, with statistically significant differences (P<0.05). Multivariate logistic regression results showed that the variables included in the regression equation of the numeration data model were age and cerebrovascular function score, and the OR (95%CI) were 1.062 (1.020-1.105） and 6.838 (1.583-29.547).@*Conclusion@#Aging, low high-density lipoprotein cholesterol level, and low cerebrovascular function score may be the risk factors affecting MRI-defined cerebral ischemia.
ABSTRACT
Objective:This study aimed to analyze the relationship between cerebral ischemia and cerebral blood flow dynamics (cerebrovascular function score), related physiological, and biochemical indexes.Methods:We selected 366 patients who underwent regular physical examination, cerebrovascular hemodynamics test, and head MRI in the health management center of Shandong Electric Power Central Hospital from May 2016 to April 2019. The patients with cerebral ischemia were selected as the case group (264 cases), and those without cerebral ischemia as the control group (102 cases) to analyze the differences in cerebrovascular function scores, blood pressure, body mass index, and related biochemical indexes between the two groups. The risk factors were also analyzed by multiple logistic regression analysis.Results:The average age of cerebral the ischemia group was (53.0±7.6) years, which consisted of 211 men and 53 women. The average age of the control group was (48.7±7.3) years, which consisted of 87 men and 15 women. The comparison of the mean of continuous variables between the two groups of subjects shows that the mean age [(53.0±7.6) years old vs. (48.7±7.3) years old] and systolic blood pressure [(127.70±17.06) mmHg vs.(122.36±14.75) mmHg] (1 mmHg=0.133 kPa) of cerebral ischemia group were significantly higher than that of control group. The high-density lipoprotein level [(1.29±0.26) mmol/L vs. (1.37±0.30) mmol/L] and cerebrovascular function score [(86.55±18.41) vs. （92.16±10.77）] were significantly lower than those in the control group. The exposure rate of systolic blood pressure ≥140 mmHg and cerebrovascular function score <75 [16.7%(44 cases) vs. 2.9%(3 cases)] in the ischemic group was significantly higher than that in the control group, with statistically significant differences ( P<0.05). Multivariate logistic regression results showed that the variables included in the regression equation of the numeration data model were age and cerebrovascular function score, and the OR (95% CI) were 1.062 (1.020-1.105） and 6.838 (1.583-29.547). Conclusion:Aging, low high-density lipoprotein cholesterol level, and low cerebrovascular function score may be the risk factors affecting MRI-defined cerebral ischemia.
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<p><b>OBJECTIVE</b>To investigate the relationship between sensitivity to cisplatin (DDP) and the expression of HSP70 in cervical cancer cells in vitro.</p><p><b>METHODS</b>Cervical cancer Hela229 cells treated with different concentrations of DDP and the HSP70 inhibitor (PFT-µ) were examined for cell viability using MTT assay and colony forming ability. The cell apoptosis was analyzed by flow cytometry with propidium iodide staining and DAPI staining, and JC-1 staining was used to determine mitochondrial membrane potential. The expressions of HSP70, Bcl-2, Bax and caspase-3 were measured with Western blotting. A nude mouse model bearing Hela229 cell xenograft was used to evaluate the effect of DDP and PFT-µ on tumor growth.</p><p><b>RESULTS</b>Hela229 cells expressed a higher level of HSP70 than normal cervical cells. The combined use of PFT-µ significantly enhanced the inhibitory effect of DDP (P<0.01) and increased the cell apoptosis in Hela229 cells. JC-1 staining demonstrated that DDP combined with PFT-µ more obviously reduced mitochondrial membrane potential. DDP combined with PFT-µ more strongly lowered Bcl-2 expression and increased the expressions of casepase-3 and Bax than DDP alone. In the nude mouse model, PFT-µ significantly enhanced DDP sensitivity of Hela229 cell xenografts (P<0.01).</p><p><b>CONCLUSIONS</b>Inhibition of HSP70 expression can enhance the sensitivity of cervical cancer cell to DDP both in vivo and in vitro possibly by promoting cell apoptosis, suggesting the potential of HSP70 as a new target for gene therapy of cervical cancer.</p>